Xinming Tong

A polymer and biomaterial scientist

NCBI ORCiD ResearchGate Google Scholar

300 Pasteur Drive, Edwards Building R110-114

Stanford, CA, 94305

xinming@stanford.edu

(650)725-0796

(650)223-5345

Education

2009, PhD, Material Science and Engineering, Beijing Institute of Technology, Beijing, China

2004, BS, Polymer Science and Engineering, Beijing Institute of Technology, Beijing, China

Experience

2018 - Present, Instructor, Stanford University, CA, US

2016 - 2018, Research Associate, Stanford University, CA, US

2011 - 2016, Postdoctoral Research fellow, Stanford University, CA, US

2009 - 2011, Postdoctoral Research fellow, Tsinghua University, Beijing, China

Links

SOM


SOM


SOM

GALLERY

Objective

I am a research scientist in the Department of Orthopaedic Surgery at Stanford. I have extensive expertise in the fields of polymer chemistry, material science and tissue engineering. I am highly motivated to pursue an academic research career to contribute my expertise to the field of biomedical research by developing novel material tools for biology and medicine.

Education

2009, PhD, Material Science and Engineering, Beijing Institute of Technology, Beijing, China

2004, BS, Polymer Science and Engineering, Beijing Institute of Technology, Beijing, China

Experience

2018 - Present, Instructor, Stanford University, CA, US

2016 - 2018, Research Associate, Stanford University, CA, US

2011 - 2016, Postdoctoral Research fellow, Stanford University, CA, US

2009 - 2011, Postdoctoral Research fellow, Tsinghua University, Beijing, China

Research Interests

  • Novel injectable biomaterials as 3D stem cell niche to enhance differentiation and tissue formation in 3D.
  • Hydrogel platforms with independently tunable biochemical and mechanical properties to decipher cell-niche interactions.
  • Polymer hydrogel as long-term stable tissue substitute
  • Strategies to synthesize vinyl copolymers with accurate control on the sequence of the monomer units.
  • Synthesis and applications of cyclodextrin based polyrotaxanes

Awards and Grants

  • RO1 NIH/NIAMS. (Co-I, 2019-)
  • Stanford SPARK Translational Research Grant (Co-PI, 2019-)
  • Stanford Child Health Research Institute Pilot Grant (PI, 2018-2019)
  • Young Scholar Grant from National Science Foundation of China (PI, 2011-2013)
  • National Postdoctoral Fellowship of China (2011)
  • Excellent Graduate Student, Beijing Institute of Technology (2009)

Publications

  1. Tong, X.; Yang, F., Recent Progress in Developing Injectable Matrices for Enhancing Cell Delivery and Tissue Regeneration. Adv. Healthc. Mater., 2018;7(7):e1701065., [View] [PMID: 29280328]
  2. Tong, X.; Yang, F., Sliding Hydrogels with Mobile Molecular Ligands and Crosslinks as 3D Stem Cell Niche. Adv. Mater., 2016, 28 (33), 7257-63. [PMID: 27305637][PMC5127628]
  3. Tong, X.; Jiang, J.; Zhu, D.; Yang, F., Hydrogels with Dual Gradients of Mechanical and Biochemical Cues for Deciphering Cell-Niche Interactions, ACS Biomater. Sci. Eng., 2016, 2, 845-852. [view]
  4. Tong, X.; Lee, S.; Bararpour, L.; Yang, F., Sustained protein release from hydrogels by tuning poly(ethylene glycol) structure and degradation, Macromol. Biosci., 2015, 15, 1679-1686.[PMID: 26259711][PMC5127624]
  5. Tong, X.; Yang, F., Interpenetrating network hydrogel as biomimetic cell niche with independently tunable biochemical and mechanical properties, Biomaterials, 2014, 35, 1807-1815.[PMID: 24331710]
  6. Tong, X.; Lai, J.; Guo, B.; Huang, Y., A new end group structure of PEG for hydrolysis- resistant biomaterials. J. Polym. Sci., Part A: Polym. Chem., 2011, 49, 1513-1516.[view]
  7. Tong, X.; Guo, B.; Huang, Y., Toward the synthesis of sequence-controlled vinyl copolymers. Chem. Comm., 2011, 47, 1455-1457.[PMID: 21125120]
  8. Tong, X.; Gao, P.; Zhang, X.; Ye, L.; Zhang, A.; Feng, Z., End-capping double-chain stranded polypseudorotaxanes using lengthily tunable poly(2-hydroxyethyl methacrylate) blocks via atom transfer radical polymerization. Polym. Int., 2010, 59, 917-922.[view]
  9. Tong, X.; Zhang, X.; Ye, L.; Zhang, A.; Feng, Z., Synthesis and characterization of block copolymers comprising a polyrotaxane middle block flanked by two brush-like PCL blocks. Soft Matter, 2009, 5, 1848-1855.[view]
  10. Tong, X.; Zhang, X.; Ye, L.; Zhang, A.; Feng, Z., Novel main-chain polyrotaxanes synthesized via ATRP of HEMA initiated with polypseudorotaxanes comprising BriB-PEG-iBBr and alpha-CDs. Polymer, 2008, 49, 4489-4493.[view]
  11. Tong, X.; Hou, D.; Zhang, X.; Ye, L.; Zhang, A.; Feng, Z., Synthesis and characterization of polyrotaxanes made from alpha-CDs threaded onto triblock copolymers with PEG as a central axle and flanked by two low molecular weight polystyrenes as outer stoppers. Chinese J. Poly. Sci., 2008, 26, 723-732. [view]
  12. Yan, X.; Yang, X.; Tong, X. *; Huang Y.*, A method to accelerate the gelation of disulfide-crosslinked hydrogels, Chinese J. Poly. Sci., 2015, 33, 118-127. [view]
  13. Song, Q., Luo, Z.; Tong, X. *; Du, Y.; Huang, Y., Glutathione as the end capper for cryclodextrin/PEG polyrotaxanes, Chinese J. Poly. Sci., 2014, 32, 1003-1009. [view]

  14. Rogan H, Ilagan F, Tong, X., Chu CR, Yang F. Microribbon-hydrogel composite scaffold accelerates cartilage regeneration in vivo with enhanced mechanical properties using mixed stem cells and chondrocytes. Biomaterials, 2020; 228:119579. [view]
  15. Stanton, AE.; Tong, X.; Yang, F. Varying solvent type modulates collagen coating and stem cell mechanotransduction on hydrogel substrates. APL Bioengineering, 2019; (doi: 10.1063/1.5111762.)[view]
  16. Stanton, AE.; Tong, X.; Lee, S.; Yang, F. Extracellular matrix type modulates mechanotransduction of stem cells. Acta Biomater, 2019; (doi: 10.1016/j.actbio.2019.06.048.)[view][PMID: 31255664]
  17. Stanton, AE.; Tong, X.; Lee, S.; Yang, F. Biochemical Ligand Density Regulates Yes-Associated Protein Translocation in Stem Cells through Cytoskeletal Tension and Integrins. ACS Appl. Mater. Interfaces,  2019;11(9):8849-57. [view][PMID: 30789697]
  18. Lee, S.; Stanton, AE.; Tong, X.; Yang, F. Hydrogels with enhanced protein conjugation efficiency reveal stiffness-induced YAP localization in stem cells depends on biochemical cues. Biomaterials, 2019;202:26-34.[view][PMID: 30826537]
  19. Zhu, D.; Tong, X..; Trinh, P.; Yang, F., Mimicking Cartilage Tissue Zonal Organization by Engineering Tissue-scale Gradient Hydrogels as 3D Cell Niche. Tissue Eng. Part A, 2018, (DOI 10.1089/ten.TEA.2016.0453. [PMID: 28385124]
  20. Lee, S.; Serpooshan, V.; Tong, X.; Venkatraman, S.; Lee, M.; Lee, J.; Chirikian, O.; Wu, J. C.; Wu, S. M.; Yang, F., Contractile force generation by 3D hiPSC-derived cardiac tissues is enhanced by rapid establishment of cellular interconnection in matrix with muscle-mimicking stiffness. Biomaterials, 2017, 131, 111-120.[view][PMID 28384492] [PMC5558787]
  21. Tevlin, R.; Seo, E. Y.; Marecic, O.; McArdle, A.; Tong, X.; Zimdahl, B.; Malkovskiy, A.; Sinha, R.; Gulati, G.; Li, X.; Wearda, T.; Morganti, R.; Lopez, M.; Ransom, R. C.; Duldulao, C. R.; Rodrigues, M.; Nguyen, A.; Januszyk, M.; Maan, Z.; Paik, K.; Yapa, K.-S.; Rajadas, J.; Wan, D. C.; Gurtner, G. C.; Snyder, M.; Beachy, P. A.; Yang, F.; Goodman, S. B.; Weissman, I. L.; Chan, C. K. F.; Longaker, M. T., Pharmacological rescue of diabetic skeletal stem cell niches. Science Translational Medicine, 2017, 9 (372). [PMID 28077677] [PMC5725192]
  22. Daldrup-Link, H. E.; Chan, C.; Lenkov, O.; Taghavigarmestani, S.; Nazekati, T.; Nejadnik, H.; Chapelin, F.; Khurana, A.; Tong, X.; Yang, F.; Pisani, L.; Longaker, M.; Gambhir, S. S., Detection of Stem Cell Transplant Rejection with Ferumoxytol MR Imaging: Correlation of MR Imaging Findings with Those at Intravital Microscopy. Radiology, 2017, 161-139. [PMID 28128708] [PMC5548449]
  23. Wang, T.; Lai, J. H.; Han, L.-H.; Tong, X.; Yang, F., Modulating stem cell-chondrocyte interactions for cartilage repair using combinatorial extracellular matrix-containing hydrogels. J. Mater. Chem. B, 2016, 4 (47), 7641-7650. [view]
  24. Wang, C.; Tong, X.; Jiang, X.; Yang, F., Effect of Matrix Metalloproteinase-Mediated Matrix Degradation on Glioblastoma Cell Behavior in 3D PEG-based Hydrogels. J. Biomed. Mater. Res. Part A, 2016, 105 (3), 770-778. [PMID 27770562] [PMC5276739]
  25. Lee, S.; Tong, X.; Yang, F., Effects of the poly(ethylene glycol) hydrogel crosslinking mechanism on protein release, Biomater. Sci., 2016, 4, 405-411. [PMID 26539660] [PMC5127629]
  26. Lee, S.; Tong, X.; Han, L.; Behn, A.; Yang, F., Aligned Microribbon-like Hydrogels for Guiding Three-dimensional Smooth Muscle Tissue Regeneration, J. Biomed. Mater. Res. Part A, 2016, 104, 1064-1071.[PMID 26799256] [PMC5127626]
  27. Deveza, L.; Ashoken, J.; Castaneda, G.; Tong, X.; Keeney, M.; Han L.H.; Yang, F., Microfluidic Synthesis of Biodegradable Polyethylene-Glycol Microspheres for Controlled Delivery of Proteins and DNA Nanoparticles, ACS Biomater. Sci. Eng., 2015, 1, 157-165. [view]
  28. Nejadnik, H.; Diecke, S.; Lenkov, O.D.; Chapelin, F.; Doing, J.; Fathi, A.; Tong, X.; Derugin, N.; Chan, R.C.; Gaur, A.; Yang, F.; Wu, J.C.; Daldrup-Link, H.E., Improved approach for chondrogenic differentiation of human induced pluripotent stem cells, Stem Cell Rev., 2015, 11, 242-253. [PMID 25578634] [PMC4412587]
  29. Lee, S.; Tong, X.; Yang, F., The effects of varying poly(ethylene glycol) hydrogel crosslinking density and crosslinking mechanism on protein accumulation in three-dimensional hydrogels, Acta Biomater., 2014, 10, 4167-4174.[PMID 24887284]
  30. Wang, C.; Tong, X.; Yang, F., Bioengineered 3D brain tumor model to elucidate the effects of matrix stiffness on glioblastoma cell behavior using PEG-based hydrogels, Mol. Pharm., 2014, 11, 2115-2125.[PMID 24712441]
  31. Wang, T.Y.; Lai, J.H.; Han, L.H.; Tong, X.; Yang, F., Chondrogenic differentiation of adipose-derived stromal cells in combinatorial hydrogels containing cartilage matrix proteins with decoupled mechanical stiffnesses, Tissue Eng. Part A., 2014, 20, 2131-2139. [PMID 24707837]
  32. Han, L.H.; Tong, X.; Yang, F., Photocrosslinkable PEG-based microribbons for forming 3D macroporous scaffolds with decoupled niche properties, Adv. Mater., 2014, 26, 1757-62.[PMID 24347028]
  33. Keeney, M.; Onyiah, S.; Zhang, Z.; Tong, X.; Han, L.H.; Yang, F., Modulating polymer chemistry to enhance non-viral gene delivery inside hydrogels with tunable matrix stiffness, Biomaterials, 2013, 34, 9657-9665.[PMID 24011715]
  34. Hammer, J.; Han, L.H.; Tong, X.; Yang, F., A Cell-friendly Process to Fabricate Hydrogels with Microchannel-like Porosity for Tissue Engineering, Tissue Eng., 2013, 20, 169-176.[PMID 23745610]
  35. Keeney, M.; Mathur, M.; Cheng, E.; Tong, X.; Yang, F., The effects of polymer end-group chemistry and order of deposition on controlled protein delivery from layer-by-layer assembly. Biomacromolecules, 2013, 14, 794-800.[view]
  36. Tao, Y.; Tong, X.; Zhang, Y.; Lai, J.; Huang, Y.; Jiang, Y.-R.; Guo, B.-H., Evaluation of an in situ chemically crosslinked hydrogel as a long-term vitreous substitute material. Acta Biomater., 2013, 9, 5022-30.[PMID 23022890]
  37. Zhang, X.; Zhu, X.; Tong, X.; Ye, L.; Zhang, A.; Feng, Z., Novel main-chain polyrotaxanes synthesized via ATRP of HPMA in aqueous media. J. Polym. Sci., Part A: Polym. Chem., 2008, 46, 5283-5293.[view]
  38. Hou, D.; *Tong, X.*; Yu, H.; Zhang, A.; Feng, Z., A kind of novel biodegradable hydrogel made from copolymerization of gelatin with polypseudorotaxanes based on alpha-CDs. Biomed. Mater., 2007, 2, S147-S152.[PMID 18458460]

Patents

  1. Han, L.; Yang, F.; Tong, X. "Macroporous 3-D scaffolds for tissue engineering". (US Patent 9,402,710 B2, 2016.)
  2. Yang, F.; Han, L.; Tong X. "Dynamic macropore formation using multiple porogens". (US Patent 9,415,138 B2, 2016)
  3. Tao, Y.; Jiang, Y.; Guo, B.; Huang, Y.; Tong, X. "Application of in-situ crosslinking hydrogel capable of intraocular injection in preparing artificial vitreous bodies". (Chinese Patent CN 101934089, 2013)
  4. Tong, X.; Guo, B.; Lai, J.; Huang, Y., "Polymer capable of performing addition reaction with thiol and having stable bonding hydrolysis". (Chinese Patent CN 101885841, 2012.)

Conference Talks

  1.  Tong, X.; Yang, F., Sliding Hydrogels with Molecular Mobility Enhance Stem Cell Chondrogenesis,  Cellular and Molecular Bioengineering (CMBE) Conference, Puerto Rico, 2020.
  2. Tong, X.; Yang, F., Sliding hydrogels with tunable molecular mobility and ligands as 3D niche for accelerating MSC-based cartilage regeneration, Biomedical Engineering Society Annual Meeting, Atlanta, GA, 2018.
  3. Tong, X.; Watkins L., Eugenis J., Yang, F., Macroprous microribbon hydrogels with tunable extracellular matrix compositions induce robust osteogenesis of mesenchymal stem cells in 3D, Biomedical Engineering Society Annual Meeting, Atlanta, GA, 2018.
  4. Tong, X.; Li, J., Yang, F., Engineering PEG-based microribbon hydrogels with independently tunable mechanical and biochemical properties to modulate osteogenesis of human mesenchymal stem cells, Biomedical Engineering Society Annual Meeting, Phoenix, AZ, 2017.
  5. Tong, X.; Yang, F., Sliding hydrogels with tunable molecular mobility and degradation enhance and accelerate neocartilage formation by mesenchymal stem cells in 3D, Biomedical Engineering Society Annual Meeting, Phoenix, AZ, 2017.
  6. Tong, X.; Yang, F., "Sliding hydrogels with mobile molecular ligands and crosslinks enhance differentiation of human mesenchymal stem cells toward multiple lineages", TERMIS-AM annual conference, San Diego, CA, 2016.
  7. Tong, X.; Yang, F., "Engineering Sliding Hydrogels with Molecular Mobility as 3D Stem Cell Niche", Biomedical Engineering Society Annual Meeting, Tampa, FL, 2015.
  8. Tong, X.; Yang, F., "Engineering 3D sliding hydrogels with mobile molecular ligands to direct stem cell differentiation", Society For Biomaterials Annual Meeting, Charlotte, NC, 2015.
  9. Tong, X.; Lee, S.; Wen, Y.; Phadnis, S.; Chen, B.; Reijo P.R.; Yang, F., "Injectable hydrogels as cell delivery matrices for smooth muscle repair", Material Research Society Annual Spring Meeting, San Francisco, CA, 2014.
  10. Huang, Y.; Tong, X.; Yang, D.; Lai J., "Is it possible to achieve monomer-level sequence control in vinyl copolymers?", 246st National Meeting and Exposition of the American Chemical Society, Indianapolis, IN, 2013.
  11. Tong, X.; Yang, F. "Engineering Stem Cell Microenvironment with independently tunable biochemical and mechanical properties", Material Research Society Annual Spring Meeting, San Francisco, CA, 2012.